Abstract
Introduction: At present, endodontic failure remains the most urgent problem of contemporary endodontics. The main reason for the complication of endodontic treatment is the retention of pathogenic microbe- Enterococcus faecalis inside the root canal system after endodontic treatment. In the era of advances in endodontics, dentists still face challenges in the decontamination of canals, as Enterococcus faecalis is resistant to the most widely used, "rated" canal disinfection agents. Therefore in modern endodontic practice, the issue of finding an effective antimicrobial solution for the root canal system irrigation is still relevant.
On other hand, in the New Millennium, the world pharmacy is becoming more and more focused on natural substances. Taking this trend into account, research and implementation of new herbal/bio antimicrobial preparations is a prerequisite for the successful management of persistent endodontic infection. The literature suggests the Plant Cotinus coggygria Scop’s (family Anacardiaceae) diverse biological activities including antimicrobial against a wide range of microorganisms. For this reason, this plant was selected for our study.
Objectives: The goal of our preclinical research was determination the antimicrobial efficacy/activity of Cotinus coggygria Scop extract water solutions against Enterococcus faecalis.
Materials and methods: At TSMU Iovel Kutateladze Institute of Pharmacochemistry unique formulation of cultivated in Georgia Cotinus coggygria Scop leaves polyphenol extract has been developed. Later extract aqueous solutions of various concentrations (2%, 5%, 10% and 15%) were prepared as endodontic irrigation solutions exclusively for our study. Microbiological study of polyphenol extract water solutions against endodontic pathogens (Streptococcus spp. and Enterococcus spp.) was conducted at the Laboratory of General Microbiology of Eliava Bacteriophage Analytical Diagnostic Center.
Results: The In vitro studies seems encouraging as 2% extract was found to be most effective against Streptococcus spp, while extract 10% solutions were observed to be most effective against Enterococcus faecalis.
Summary and Conclusion: Pronounced antibacterial activity suggests successful application of Cotinus coggygria Scop extract solutions in Endodontic Practice. The in vivo study is in progress for recommending this novel herbal/bio preparation as an alternative antimicrobial endodontic irrigant.